| 质粒名称: | pET co-transformation cloning vector (13S-A) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 48323 |
| 存储实验室: | Scott Gradia |
|
载体骨架基本信息 |
|
| 载体名称: | pET |
| 载体出品公司: | Clontech |
| 空载体大小: | 3566 bp |
| 完整质粒大小: | 3566 bp |
| 载体类型: | 细菌细胞表达 |
| 载体抗性: | Spectinomycin, 50 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | XL1 Blue |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | 无 |
|
文献引用方法 |
|
| 材料和方法部分: | pET co-transformation cloning vector (13S-A) was a gift from Scott Gradia (Addgene plasmid # 48323 ; http://n2t.net/addgene:48323 ; RRID:Addgene_48323) |
买家导航
pET co-transformation cloning vector (13S-A)载体质粒基本信息
pET co-transformation cloning vector (13S-A)质粒图谱载体图谱和pET co-transformation cloning vector (13S-A)载体序列质粒序列多克隆位点信息
pET co-transformation cloning vector (13S-A)质粒载体简介
pET co-transformation cloning vector (13S-A)质粒序列载体序列
LOCUS pET co-transformation cloning vector (13S-A) 3566 bp DNA circular SYN 14-APR-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3566)
TITLE Series-13: Co-transformation vectors for Series-2 transfer vectors
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 3566)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT Sequence Label: pET co-transformation cloning vector (13S-A)
FEATURES Location/Qualifiers
source 1..3566
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(3..1085)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
primer_bind 1047..1066
/label=LacI-R
/note="LacI, reverse primer"
promoter complement(1086..1163)
/gene="lacI"
/label=lacI promoter
primer_bind 1209..1228
/label=T7
/note="T7 promoter, forward primer"
promoter 1209..1227
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 1232..1255
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 1308..1330
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
primer_bind complement(1458..1476)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 1472..1519
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
CDS complement(1690..2481)
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
promoter complement(2482..2573)
/gene="bla"
/label=AmpR promoter
rep_origin complement(2621..3359)
/direction=LEFT
/label=CloDF13 ori
/note="Plasmids containing the CloDF13 (CDF) origin of
replication can be propagated in E. coli cells that contain
additional plasmids with compatible origins."
ORIGIN
1 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
61 aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt
121 gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg
181 tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata
241 taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatgtccgc accaacgcgc
301 agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc
361 atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg
421 gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta
481 tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg
541 atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg
601 gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca
661 ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc
721 agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg
781 cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc
841 gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc
901 aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc
961 atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg
1021 cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt
1081 ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag
1141 gttttgcgcc attcgatggt gtccgggatc tcgacgctct cccttatgcg actcctgcat
1201 taggaaatta atacgactca ctatagggag agaattgtga gcggataaca attcccctgt
1261 agattaatta agcggccgcc ctgcaggact cgagttctag aaataatttt gtttaacttt
1321 aagaaggaga tatagatatc ccaactccat aaggatccgc gatcgcggcg cgccacctgg
1381 tggccggccg gtaccacgcg tgcgcgctga tccggctgct aacaaagccc gaaaggaagc
1441 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
1501 ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg tcacactgct
1561 tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt aacgaccctg
1621 ccctgaaccg acgaccgggt catcgtggcc ggatcttgcg gcccctcggc ttgaacgaat
1681 tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtaat ggacaaattc
1741 ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat aagcctgtct
1801 agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc
1861 gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag
1921 cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc
1981 atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg
2041 acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc
2101 gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt ctccaaattg
2161 cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac
2221 ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt
2281 gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat
2341 atcactgtgc ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa
2401 cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc
2461 ggcgatcacc gcttccctca tactcttcct ttttcattat tattgaagca tttatcaggg
2521 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaatagctag
2581 ctcactcggt cgctacgctc cgggcgtgag actgcggcgg gcgctgcgga cacatacaaa
2641 gttacccaca gattccgtgg ataagcaggg gactaacatg tgaggcaaaa cagcagggcc
2701 gcgccggtgg cgtttttcca taggctccgc cctcctgcca gagttcacat aaacagacgc
2761 ttttccggtg catctgtggg agccgtgagg ctcaaccatg aatctgacag tacgggcgaa
2821 acccgacagg acttaaagat ccccaccgtt tccggcgggt cgctccctct tgcgctctcc
2881 tgttccgacc ctgccgttta ccggatacct gttccgcctt tctcccttac gggaagtgtg
2941 gcgctttctc atagctcaca cactggtatc tcggctcggt gtaggtcgtt cgctccaagc
3001 tgggctgtaa gcaagaactc cccgttcagc ccgactgctg cgccttatcc ggtaactgtt
3061 cacttgagtc caacccggaa aagcacggta aaacgccact ggcagcagcc attggtaact
3121 gggagttcgc agaggatttg tttagctaaa cacgcggttg ctcttgaagt gtgcgccaaa
3181 gtccggctac actggaagga cagatttggt tgctgtgctc tgcgaaagcc agttaccacg
3241 gttaagcagt tccccaactg acttaacctt cgatcaaacc acctccccag gtggtttttt
3301 cgtttacagg gcaaaagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
3361 tttctactga accgctcttg atttcagtgc aatttatctc ttcaaatgta gcacctgaag
3421 tcagccccat acgatataag ttgtaattct catgttagtc atgccccgcg cccaccggaa
3481 ggagctgact gggttgaagg ctctcaaggg catcggtcga gatcccggtg cctaatgagt
3541 gagctaactt acattaattg cgttgc
//
